alpha 2 Antiplasmin ELISA 试剂盒
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- 抗原 See all alpha 2 Antiplasmin (SERPINF2) ELISA试剂盒
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.813-500 ng/mL
- 最低检测浓度
- 7.813 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 1.85 ng/mL
- 组件
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Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1× - 试剂未包括
- Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.
- Top Product
- Discover our top product SERPINF2 ELISA Kit
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Alpha2 Antiplasmin (SERPINF2) ELISA Kit
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- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all alpha 2 Antiplasmin (SERPINF2) ELISA试剂盒
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- 别名
- Alpha-2-Plasmin Inhibitor (a2PI) (SERPINF2 产品)
- 别名
- A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, serpin ELISA Kit, AI747498 ELISA Kit, Pli ELISA Kit, Serpimf2 ELISA Kit, serpinf2 ELISA Kit, serpinf2l ELISA Kit, wu:fd57d08 ELISA Kit, Dmrs91 ELISA Kit, Pedf ELISA Kit, serpin family F member 2 ELISA Kit, serpin family F member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 2 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a ELISA Kit, SERPINF2 ELISA Kit, Serpinf2 ELISA Kit, SERPINF1 ELISA Kit, serpinf2a ELISA Kit, Serpinf1 ELISA Kit
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
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