FLT1 ELISA 试剂盒
-
- 抗原 See all FLT1 ELISA试剂盒
- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
-
适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.41 ng/mL
- 最低检测浓度
- 0.41 ng/mL
- 应用范围
- ELISA
- 原理
- Rat VEGFR1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects Rat VEGFR1 and Mouse VEGFR1. Other species not determined.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling FLT1 ELISA Kit
- Top Product
- Discover our top product FLT1 ELISA Kit
-
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA Kit
FLT1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 人 Colorimetric Sandwich ELISA 1.5-400 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
-
Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all FLT1 ELISA试剂盒
- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
- 别名
- VEGFR1 (FLT1 产品)
- 别名
- FLT ELISA Kit, FLT-1 ELISA Kit, VEGFR-1 ELISA Kit, VEGFR1 ELISA Kit, FLT1 ELISA Kit, flt1b ELISA Kit, sFlt1 ELISA Kit, AI323757 ELISA Kit, Flt-1 ELISA Kit, fms related tyrosine kinase 1 ELISA Kit, FMS-related tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor 1 ELISA Kit, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit, FMS-like tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor-1 ELISA Kit, FLT1 ELISA Kit, Flt1 ELISA Kit, CpipJ_CPIJ012087 ELISA Kit, flt1 ELISA Kit, FLT-1 ELISA Kit
- 背景
- Vascular Endothelial Growth Factor Receptor 1 (Flt1)
- 基因ID
- 54251
- UniProt
- P53767
- 途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
-
