UNC5A ELISA 试剂盒
UNC5A
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all UNC5A ELISA试剂盒
- UNC5A (Unc-5 Homolog A (UNC5A))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat UNC5H1 (Netrin Receptor UNC5A) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Rat UNC5H1. Other species not yet determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product UNC5A ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all UNC5A ELISA试剂盒
- UNC5A (Unc-5 Homolog A (UNC5A))
- 别名
- UNC5H1 (UNC5A 产品)
- 别名
- zgc:175190 ELISA Kit, UNC5H1 ELISA Kit, Unc5h1 ELISA Kit, mKIAA1976 ELISA Kit, unc-5 netrin receptor A ELISA Kit, netrin receptor UNC5A ELISA Kit, UNC5A ELISA Kit, Tsp_02512 ELISA Kit, Tsp_13881 ELISA Kit, unc5a ELISA Kit, Unc5a ELISA Kit
- 背景
- Netrin receptor UNC5A (Protein unc-5 homolog 1) (Protein unc-5 homolog A)
- 基因ID
- 60629
- UniProt
- O08721
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