PTK2B ELISA 试剂盒
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- 抗原 See all PTK2B ELISA试剂盒
- PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
- 抗原表位
- pTyr402, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit. This assay semi-quantitatively measures phophorylated PYK2 (Tyr402) and Total PYK2 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes PYK2 phosphorylated at Tyrosine-402 as well as total PYK2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PTK2B ELISA Kit
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PTK2B Protein tyrosine Kinase 2 beta (PTK2B) ELISA Kit
PTK2B 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
PTK2B Protein tyrosine Kinase 2 beta (PTK2B) ELISA KitPTK2B 适用: 人 pTyr402 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.Add 100 μL of sample or positive control to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared primary antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared 1X HRP-Streptavidin to each well.Incubate 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all PTK2B ELISA试剂盒
- PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
- 别名
- PYK2 (PTK2B 产品)
- 别名
- CADTK ELISA Kit, CAKB ELISA Kit, FADK2 ELISA Kit, FAK2 ELISA Kit, PKB ELISA Kit, PTK ELISA Kit, PYK2 ELISA Kit, RAFTK ELISA Kit, CAKbeta ELISA Kit, E430023O05Rik ELISA Kit, Raftk ELISA Kit, Pyk2 ELISA Kit, fak1b ELISA Kit, ptk2.2 ELISA Kit, ptk2b ELISA Kit, si:ch1073-355e8.1 ELISA Kit, protein tyrosine kinase 2 beta ELISA Kit, PTK2 protein tyrosine kinase 2 beta ELISA Kit, protein tyrosine kinase 2aa ELISA Kit, PTK2B ELISA Kit, Ptk2b ELISA Kit, ptk2aa ELISA Kit
- 背景
- Protein tyrosine kinase 2 beta, PTK2B, CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK
- 基因ID
- 2185
- UniProt
- Q14289
- 途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
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