Reticulon 4 ELISA 试剂盒
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- 抗原 See all Reticulon 4 (RTN4) ELISA试剂盒
- Reticulon 4 (RTN4)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Reticulon 4 (RTN4) ELISA Kit
RTN4 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Reticulon 4 (RTN4) ELISA KitRTN4 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Reticulon 4 (RTN4) ELISA KitRTN4 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Reticulon 4 (RTN4) ELISA KitRTN4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Reticulon 4 (RTN4) ELISA试剂盒
- Reticulon 4 (RTN4)
- 别名
- Nogo-A (RTN4 产品)
- 别名
- ASY ELISA Kit, NI220/250 ELISA Kit, NOGO ELISA Kit, NOGO-A ELISA Kit, NOGOC ELISA Kit, NSP ELISA Kit, NSP-CL ELISA Kit, Nbla00271 ELISA Kit, Nbla10545 ELISA Kit, Nogo-B ELISA Kit, Nogo-C ELISA Kit, RTN-X ELISA Kit, RTN4-A ELISA Kit, RTN4-B1 ELISA Kit, RTN4-B2 ELISA Kit, RTN4-C ELISA Kit, RTN4-Cw ELISA Kit, DKFZp459C0314 ELISA Kit, 1110020G17Rik ELISA Kit, AA407876 ELISA Kit, AA409940 ELISA Kit, AA960376 ELISA Kit, C130026I10Rik ELISA Kit, NgA ELISA Kit, Nogo-A ELISA Kit, mKIAA0886 ELISA Kit, mKIAA4153 ELISA Kit, NI-250 ELISA Kit, Nogo ELISA Kit, Vp20 ELISA Kit, r ELISA Kit, rat N ELISA Kit, rat NogoA ELISA Kit, reticulon-4 ELISA Kit, nogo ELISA Kit, rtn4 ELISA Kit, wu:fb17a02 ELISA Kit, wu:fb22f12 ELISA Kit, wu:fd61a07 ELISA Kit, zgc:92163 ELISA Kit, reticulon 4 ELISA Kit, reticulon 4a ELISA Kit, RTN4 ELISA Kit, rtn4 ELISA Kit, Rtn4 ELISA Kit, rtn4a ELISA Kit
- 背景
- Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)
- 基因ID
- 83765
- UniProt
- Q9JK11
- 途径
- Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome
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