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Reticulon 4 ELISA 试剂盒

RTN4 适用: 大鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN2748436
发货至: 中国
  • 抗原 See all Reticulon 4 (RTN4) ELISA试剂盒
    Reticulon 4 (RTN4)
    适用
    • 4
    • 2
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Reticulon 4 (RTN4) ELISA试剂盒
    Reticulon 4 (RTN4)
    别名
    Nogo-A (RTN4 产品)
    别名
    ASY ELISA Kit, NI220/250 ELISA Kit, NOGO ELISA Kit, NOGO-A ELISA Kit, NOGOC ELISA Kit, NSP ELISA Kit, NSP-CL ELISA Kit, Nbla00271 ELISA Kit, Nbla10545 ELISA Kit, Nogo-B ELISA Kit, Nogo-C ELISA Kit, RTN-X ELISA Kit, RTN4-A ELISA Kit, RTN4-B1 ELISA Kit, RTN4-B2 ELISA Kit, RTN4-C ELISA Kit, RTN4-Cw ELISA Kit, DKFZp459C0314 ELISA Kit, 1110020G17Rik ELISA Kit, AA407876 ELISA Kit, AA409940 ELISA Kit, AA960376 ELISA Kit, C130026I10Rik ELISA Kit, NgA ELISA Kit, Nogo-A ELISA Kit, mKIAA0886 ELISA Kit, mKIAA4153 ELISA Kit, NI-250 ELISA Kit, Nogo ELISA Kit, Vp20 ELISA Kit, r ELISA Kit, rat N ELISA Kit, rat NogoA ELISA Kit, reticulon-4 ELISA Kit, nogo ELISA Kit, rtn4 ELISA Kit, wu:fb17a02 ELISA Kit, wu:fb22f12 ELISA Kit, wu:fd61a07 ELISA Kit, zgc:92163 ELISA Kit, reticulon 4 ELISA Kit, reticulon 4a ELISA Kit, RTN4 ELISA Kit, rtn4 ELISA Kit, Rtn4 ELISA Kit, rtn4a ELISA Kit
    背景
    Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)
    基因ID
    83765
    UniProt
    Q9JK11
    途径
    Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome
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