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Follistatin ELISA 试剂盒

FST 适用: 猕猴 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN2748110
发货至: 中国
  • 抗原 See all Follistatin (FST) ELISA试剂盒
    Follistatin (FST)
    适用
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    猕猴
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Rhesus Macaque Follistatin (FST) ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Rhesus Macaque Follistatin (FST)
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Follistatin (FST) ELISA试剂盒
    Follistatin (FST)
    别名
    Follistatin (FST 产品)
    别名
    CG12955 ELISA Kit, CG12956 ELISA Kit, CG33466 ELISA Kit, Dmel\\CG33466 ELISA Kit, Fol1 ELISA Kit, dFS ELISA Kit, dFol1 ELISA Kit, dfs ELISA Kit, fs ELISA Kit, FS ELISA Kit, foll ELISA Kit, xfs ELISA Kit, FST ELISA Kit, fst ELISA Kit, AL033346 ELISA Kit, FOL1 ELISA Kit, Fst-288 ELISA Kit, RATFOL1 ELISA Kit, cb446 ELISA Kit, fd07h10 ELISA Kit, fst1 ELISA Kit, wu:fd07h10 ELISA Kit, Follistatin ELISA Kit, follistatin ELISA Kit, follistatin L homeolog ELISA Kit, follistatin a ELISA Kit, Fs ELISA Kit, fst ELISA Kit, FST ELISA Kit, LOC100141662 ELISA Kit, fst.L ELISA Kit, Fst ELISA Kit, fsta ELISA Kit
    背景
    Follistatin (FST)
    基因ID
    707420
    UniProt
    F7AVH0
    途径
    Negative Regulation of Hormone Secretion
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