FAP ELISA 试剂盒
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- 抗原 See all FAP ELISA试剂盒
- FAP (Fibroblast Activation Protein, alpha (FAP))
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适用
- 猕猴
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rhesus Macaque Fibroblast Activation Protein (FAP) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rhesus Macaque Fibroblast Activation Protein (FAP)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product FAP ELISA Kit
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Fibroblast Activation Protein, alpha (FAP) ELISA Kit
FAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 AA 26-760 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 12-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all FAP ELISA试剂盒
- FAP (Fibroblast Activation Protein, alpha (FAP))
- 别名
- FAP (FAP 产品)
- 别名
- fap-A ELISA Kit, gene 13 ELISA Kit, FAP ELISA Kit, fapa ELISA Kit, dppiv ELISA Kit, seprase ELISA Kit, DPPIV ELISA Kit, FAPA ELISA Kit, fibroblast activation protein ELISA Kit, fibroblast activation protein alpha L homeolog ELISA Kit, fibroblast activation protein alpha ELISA Kit, fibroblast activation protein, alpha ELISA Kit, prolyl endopeptidase FAP ELISA Kit, Fap ELISA Kit, fap.L ELISA Kit, FAP ELISA Kit, fap ELISA Kit, LOC100547273 ELISA Kit
- 背景
- Fibroblast Activation Protein (FAP)
- 基因ID
- 701541
- UniProt
- F7EF58
- 途径
- Tube Formation
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