DKK1 ELISA 试剂盒
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- 抗原 See all DKK1 ELISA试剂盒
- DKK1 (Dickkopf Homolog 1 (DKK1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat Dickkopf-1 (DKK1) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rat Dickkopf-1 (DKK1)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Dickkopf Homolog 1 (DKK1) ELISA Kit
DKK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 AA 30-272 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 AA 32-266 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 AA 23-270 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all DKK1 ELISA试剂盒
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 别名
- Dkk-1 (DKK1 产品)
- 别名
- DKK-1 ELISA Kit, SK ELISA Kit, AI414495 ELISA Kit, SGY ELISA Kit, SGY-1 ELISA Kit, SGY1 ELISA Kit, Soggy ELISA Kit, mSgy ELISA Kit, dkk1 ELISA Kit, dkk ELISA Kit, dkk-1 ELISA Kit, xdkk1 ELISA Kit, Xdkk-1 ELISA Kit, Dickkopf1 ELISA Kit, dickkopf-1 ELISA Kit, mdkk-1 ELISA Kit, dkk1-A ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 ELISA Kit, dickkopf like acrosomal protein 1 ELISA Kit, dickkopf-related protein 1 ELISA Kit, dickkopf-like 1 ELISA Kit, dickkopf WNT signaling pathway inhibitor 1b ELISA Kit, dickkopf protein ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 S homeolog ELISA Kit, DKK1 ELISA Kit, Dkk1 ELISA Kit, DKKL1 ELISA Kit, LOC100228789 ELISA Kit, Dkkl1 ELISA Kit, dkk1b ELISA Kit, dkk1 ELISA Kit, dickkopf ELISA Kit, dkk1.S ELISA Kit
- 背景
- Dickkopf-1 (DKK1)
- 基因ID
- 100912050, 293897
- UniProt
- D3Z9J1
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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