CD80 ELISA 试剂盒
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- 抗原 See all CD80 ELISA试剂盒
- CD80
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.23-300 ng/mL
- 最低检测浓度
- 1.23 ng/mL
- 应用范围
- ELISA
- 原理
- Rat CD80 (B7-1) ELISA Kit for serum, plasma, and cell culture supernatant samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat Activin A, Adiponectin, SLAMF2, CTACK, Decorin, Eotaxin-1, EphA5, EPO, FGF-BP, Flt-3L, Galectin-1, Galectin-3, Gas-1, GFRalpha-1, gp-130, HGF, IL-1R6, IL-1ra, IL-2Ralpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1 (KIM-1), TIMP-2, TREM-1, TWEAK R.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product CD80 ELISA Kit
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CD80 (CD80) ELISA Kit
CD80 适用: 人 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 AA 37-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 大鼠 AA 39-248 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 人 AA 35-242 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 Colorimetric Sandwich ELISA 28-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 大鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CD80 ELISA试剂盒
- CD80
- 别名
- CD80 (CD80 产品)
- 别名
- B7 ELISA Kit, B7-1 ELISA Kit, B7.1 ELISA Kit, BB1 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Kit, LAB7 ELISA Kit, B71 ELISA Kit, Cd28l ELISA Kit, Ly-53 ELISA Kit, Ly53 ELISA Kit, MIC17 ELISA Kit, TSA1 ELISA Kit, CD80 molecule ELISA Kit, Cd80 molecule ELISA Kit, CD80 antigen ELISA Kit, CD80 ELISA Kit, Cd80 ELISA Kit
- 背景
- CD80 (B7-1)
- UniProt
- Q62680
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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