BTK ELISA 试剂盒
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- 抗原 See all BTK ELISA试剂盒
- BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))
- 抗原表位
- pTyr551
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-BTK (Y551) ELISA Kit. This assay semi-quantitatively measures phophorylated BTK (Tyr551) in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human, mouse, and rat MKK6 phosphorylated at site Serine-207 as well as total MKK6.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA Kit
BTK 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all BTK ELISA试剂盒
- BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))
- 别名
- BTK (BTK 产品)
- 别名
- BTK ELISA Kit, atk ELISA Kit, bpk ELISA Kit, xla ELISA Kit, imd1 ELISA Kit, agmx1 ELISA Kit, psctk1 ELISA Kit, AGMX1 ELISA Kit, AT ELISA Kit, ATK ELISA Kit, BPK ELISA Kit, IMD1 ELISA Kit, PSCTK1 ELISA Kit, XLA ELISA Kit, AI528679 ELISA Kit, xid ELISA Kit, Bruton tyrosine kinase ELISA Kit, Bruton agammaglobulinemia tyrosine kinase ELISA Kit, BTK ELISA Kit, btk ELISA Kit, Btk ELISA Kit
- 背景
- Bruton's tyrosine kinase (BTK) phosphorylated at Tyrosine-551
- 基因ID
- 1133
- UniProt
- Q06187
- 途径
- Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling
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