AXL ELISA 试剂盒
-
- 抗原 See all AXL ELISA试剂盒
- AXL (AXL Receptor tyrosine Kinase (AXL))
- 抗原表位
- pTyr779
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-AXL (Y779) ELISA Kit. This assay semi-quantitatively measures phophorylated AXL (Tyr779) in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human AXL phosphorylated at site Y779 .
- 产品特性
-
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling AXL ELISA Kit
- Top Product
- Discover our top product AXL ELISA Kit
-
AXL Receptor tyrosine Kinase (AXL) ELISA Kit
AXL 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 大鼠 Colorimetric Sandwich ELISA 2.4 ng/mL - 150 ng/mL Cell Lysate, Tissue Homogenate
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 人 Colorimetric Sandwich ELISA 2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 小鼠 AA 20-443 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 人 AA 33-440 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 小鼠 Colorimetric Sandwich ELISA 1-250 pg/mL Cell Culture Supernatant, Plasma, Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
AXL Receptor tyrosine Kinase (AXL) ELISA KitAXL 适用: 小鼠 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
-
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all AXL ELISA试剂盒
- AXL (AXL Receptor tyrosine Kinase (AXL))
- 别名
- AXL (AXL 产品)
- 别名
- JTK11 ELISA Kit, UFO ELISA Kit, AI323647 ELISA Kit, Ark ELISA Kit, Tyro7 ELISA Kit, Ufo ELISA Kit, AXL receptor tyrosine kinase ELISA Kit, Axl receptor tyrosine kinase ELISA Kit, AXL ELISA Kit, Axl ELISA Kit
- 背景
- Tyrosine-protein kinase receptor UFO (AXL) phosphorylated at Y779
- 基因ID
- 905
- UniProt
- P30530
- 途径
- RTK signaling, Cellular Response to Molecule of Bacterial Origin
-
