Angiopoietin 1 ELISA 试剂盒
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- 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
- Angiopoietin 1 (ANGPT1)
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适用
- 人, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.4-100 ng/mL
- 最低检测浓度
- 0.4 ng/mL
- 应用范围
- ELISA
- 原理
- Rat Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects rat angiopoietin-1 and human angiopoietin-1. Other species not determined.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Angiopoietin 1 (ANGPT1) ELISA Kit
ANGPT1 适用: 人 Colorimetric Sandwich ELISA 1.25-80 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitSmall Sample ANGPT1 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 小鼠 AA 16-497 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 大鼠 AA 16-498 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 人 AA 16-498 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
- Angiopoietin 1 (ANGPT1)
- 别名
- Angiopoietin-1 (ANGPT1 产品)
- 别名
- AGP1 ELISA Kit, AGPT ELISA Kit, ANG1 ELISA Kit, 1110046O21Rik ELISA Kit, Ang-1 ELISA Kit, Ang1 ELISA Kit, Agpt ELISA Kit, Agpt1 ELISA Kit, ang1 ELISA Kit, wu:fc76g06 ELISA Kit, ANG-1 ELISA Kit, angpt1 ELISA Kit, MGC80788 ELISA Kit, angiopoietin 1 ELISA Kit, angiopoietin 1 S homeolog ELISA Kit, ANGPT1 ELISA Kit, Angpt1 ELISA Kit, angpt1 ELISA Kit, angpt1.S ELISA Kit
- 背景
- Angiopoietin-1 (ANG-1)
- 基因ID
- 89807
- UniProt
- O35460
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process
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