ZAP70 ELISA 试剂盒
ZAP70
适用: 人
pTyr493
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all ZAP70 ELISA试剂盒
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- 抗原表位
- pTyr493, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) and Total ZAP70 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493 and Total ZAP70.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kit
ZAP70 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
-
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all ZAP70 ELISA试剂盒
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- 别名
- ZAP70 (ZAP70 产品)
- 别名
- SRK ELISA Kit, STCD ELISA Kit, STD ELISA Kit, TZK ELISA Kit, ZAP-70 ELISA Kit, 70kDa ELISA Kit, AI327364 ELISA Kit, Srk ELISA Kit, mrtle ELISA Kit, mur ELISA Kit, zap70 ELISA Kit, MGC80670 ELISA Kit, MGC88962 ELISA Kit, ZAP70 ELISA Kit, zgc:110383 ELISA Kit, zeta chain of T-cell receptor associated protein kinase 70 ELISA Kit, putative tyrosine-protein kinase zap-70 ELISA Kit, zeta-chain (TCR) associated protein kinase ELISA Kit, zeta chain of T cell receptor associated protein kinase 70 ELISA Kit, zeta-chain (TCR) associated protein kinase 70kDa ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa ELISA Kit, tyrosine-protein kinase ZAP-70 ELISA Kit, ZAP70 ELISA Kit, Smp_149470 ELISA Kit, Zap70 ELISA Kit, zap70.L ELISA Kit, zap70 ELISA Kit, LOC707555 ELISA Kit, LOC100342021 ELISA Kit, LOC100390922 ELISA Kit, LOC100542877 ELISA Kit
- 背景
- Zeta-chain associated protein kinase 70 (ZAP70) phosphorylated at Tyrosine-493 and Total ZAP70
- 基因ID
- 22637
- UniProt
- P43403
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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