Src ELISA 试剂盒
Src
适用: 人
pTyr419
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all Src ELISA试剂盒
- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
- 抗原表位
- pTyr419, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-SRC (Y419) and Total SRC ELISA Kit. This assay semi-quantitatively measures phophorylated SRC (Tyr419) and Total SRC in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human SRC phosphorylated at site Tyrosin-419 and Total SRC
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our top product Src ELISA Kit
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Proto-oncogene tyrosine-protein kinase Src (Src) ELISA Kit
Src 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
-
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Src ELISA试剂盒
- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
- 别名
- SRC (Src 产品)
- 别名
- AW259666 ELISA Kit, pp60c-src ELISA Kit, C-src1 ELISA Kit, CG7524 ELISA Kit, D-Src64B ELISA Kit, D-src ELISA Kit, DSRC64 ELISA Kit, DSrc ELISA Kit, DSrc64 ELISA Kit, DSrc64B ELISA Kit, Dm SRC1 ELISA Kit, DmHD-358 ELISA Kit, Dmel\CG7524 ELISA Kit, Dsrc ELISA Kit, Dsrc64 ELISA Kit, Dsrc64B ELISA Kit, HD-358 ELISA Kit, MRE5 ELISA Kit, SRC 64B ELISA Kit, Src ELISA Kit, Src1 ELISA Kit, Src64 ELISA Kit, c-src ELISA Kit, dSrc ELISA Kit, dsrc ELISA Kit, src ELISA Kit, src1 ELISA Kit, src64 ELISA Kit, src64B ELISA Kit, src64b ELISA Kit, ASV ELISA Kit, SRC1 ELISA Kit, c-SRC ELISA Kit, p60-Src ELISA Kit, c-Src ELISA Kit, PP60C-SCR ELISA Kit, SDR ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase ELISA Kit, uncharacterized LOC5574802 ELISA Kit, proto-oncogene tyrosine-protein kinase src ELISA Kit, Rous sarcoma oncogene ELISA Kit, Src oncogene at 64B ELISA Kit, SRC ELISA Kit, LOC5574802 ELISA Kit, CpipJ_CPIJ010659 ELISA Kit, Src ELISA Kit, Src64B ELISA Kit
- 物质类
- Viral Protein
- 背景
- Phospho-SRC (Y419). Full Name = Proto-oncogene tyrosine-protein kinase Src (P12931)
- 基因ID
- 6714
- UniProt
- P12931
- 途径
- JAK/STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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