SMAD1 ELISA 试剂盒
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- 抗原 See all SMAD1 ELISA试剂盒
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
- 抗原表位
- pSer463, pSer465, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-SMAD1 (SER463/465) and Total SMAD1 Kit. This assay semi-quantitatively measures phophorylated SMAD1 (Ser463/465) and Total SMAD1 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human SMAD1 phosphorylated at site Serine-463/465 and Total SMAD1.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling SMAD1 ELISA Kit
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- Discover our top product SMAD1 ELISA Kit
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SMAD, Mothers Against DPP Homolog 1 (SMAD1) ELISA Kit
SMAD1 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 1 (SMAD1) ELISA KitSMAD1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 1 (SMAD1) ELISA KitSMAD1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 1 (SMAD1) ELISA KitSMAD1 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all SMAD1 ELISA试剂盒
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
- 别名
- SMAD1 (SMAD1 产品)
- 别名
- MLP ELISA Kit, Xmad1 ELISA Kit, Xsmad1 ELISA Kit, bsp1 ELISA Kit, jv4-1 ELISA Kit, jv41 ELISA Kit, madh1 ELISA Kit, madr1 ELISA Kit, smad-1 ELISA Kit, MADH1 ELISA Kit, fb39h09 ELISA Kit, wu:fb39h09 ELISA Kit, wu:fb97e04 ELISA Kit, 2/23 ELISA Kit, CG12399 ELISA Kit, Dmel\\CG12399 ELISA Kit, E(zen)2 ELISA Kit, En(vvl) ELISA Kit, MAD ELISA Kit, Mat ELISA Kit, P-Mad ELISA Kit, P-mad ELISA Kit, PMad ELISA Kit, Smad ELISA Kit, Smad1 ELISA Kit, apg ELISA Kit, c28 ELISA Kit, dMAD ELISA Kit, dMad ELISA Kit, l(2)K00237 ELISA Kit, l(2)k00237 ELISA Kit, mad ELISA Kit, p-Mad ELISA Kit, pMAD ELISA Kit, pMad ELISA Kit, pSmad ELISA Kit, BSP-1 ELISA Kit, BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADR1 ELISA Kit, AI528653 ELISA Kit, Madh1 ELISA Kit, Madr1 ELISA Kit, Mlp1 ELISA Kit, MusMLP ELISA Kit, smad1 ELISA Kit, xmad ELISA Kit, SMAD family member 1 ELISA Kit, Mothers against dpp ELISA Kit, SMAD family member 1 L homeolog ELISA Kit, smad1 ELISA Kit, SMAD1 ELISA Kit, Mad ELISA Kit, Smad1 ELISA Kit, smad1.L ELISA Kit
- 背景
- Mothers against decapentaplegic homolog 1 (SMAD1) phosphorylated at Serine-463/465 and Total SMAD1
- 基因ID
- 4086
- UniProt
- Q15797
- 途径
- Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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