FLT4 ELISA 试剂盒
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- 抗原 See all FLT4 ELISA试剂盒
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.2-50 ng/mL
- 最低检测浓度
- 0.2 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse VEGFR3 ELISA Kit for Cell Culture Supernatants, EDTA and/or Citrate treated plasma, and Serum samples. Heparin is not recommended.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Mouse Vascular endothelial growth factor receptor 3.
- 灵敏度
- 0.2 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling FLT4 ELISA Kit
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- Discover our top product FLT4 ELISA Kit
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Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kit
FLT4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitHigh Sensitivity FLT4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 大鼠 AA 25-770 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 AA 25-770 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 15-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all FLT4 ELISA试剂盒
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
- 别名
- VEGFR3 (FLT4 产品)
- 别名
- FLT41 ELISA Kit, LMPH1A ELISA Kit, PCL ELISA Kit, VEGFR3 ELISA Kit, AI323512 ELISA Kit, Chy ELISA Kit, Flt-4 ELISA Kit, VEGFR-3 ELISA Kit, Vegfr3 ELISA Kit, cb1059 ELISA Kit, fms related tyrosine kinase 4 ELISA Kit, FMS-like tyrosine kinase 4 ELISA Kit, fms-related tyrosine kinase 4 ELISA Kit, FLT4 ELISA Kit, Flt4 ELISA Kit, flt4 ELISA Kit
- 背景
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Gene Names: Flt4 Flt-4 Vegfr3
Protein names: Vascular endothelial growth factor receptor 3 (VEGFR-3) (EC 2.7.10.1) (Fms-like tyrosine kinase 4) (FLT-4) (Tyrosine-protein kinase receptor FLT4)
- 基因ID
- 14257
- UniProt
- P35917
- 途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
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