alpha 2 Antiplasmin ELISA 试剂盒
-
- 抗原 See all alpha 2 Antiplasmin (SERPINF2) ELISA试剂盒
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.12-30 ng/mL
- 最低检测浓度
- 0.12 ng/mL
- 应用范围
- ELISA
- 原理
- Human SERPINF2 (Alpha-2-Antiplasmin) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes human SERPINF2. Other species not yet determined.
- 灵敏度
- 0.12 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SERPINF2 ELISA Kit
- Top Product
- Discover our top product SERPINF2 ELISA Kit
-
-
- 应用备注
- Recommended Dilution for serum and plasma samples200 - 2,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all alpha 2 Antiplasmin (SERPINF2) ELISA试剂盒
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- 别名
- Serpin F2 (SERPINF2 产品)
- 别名
- A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, serpin ELISA Kit, AI747498 ELISA Kit, Pli ELISA Kit, Serpimf2 ELISA Kit, serpinf2 ELISA Kit, serpinf2l ELISA Kit, wu:fd57d08 ELISA Kit, Dmrs91 ELISA Kit, Pedf ELISA Kit, serpin family F member 2 ELISA Kit, serpin family F member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 2 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a ELISA Kit, SERPINF2 ELISA Kit, Serpinf2 ELISA Kit, SERPINF1 ELISA Kit, serpinf2a ELISA Kit, Serpinf1 ELISA Kit
- 背景
-
Gene Names: SERPINF2 AAP PLI
Protein names: Alpha-2-antiplasmin (Alpha-2-AP) (Alpha-2-plasmin inhibitor) (Alpha-2-PI) (Serpin F2)
- 基因ID
- 5345
- UniProt
- P08697
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
-