CCL5 ELISA 试剂盒
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- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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适用
- Cat
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.7-200 pg/mL
- 最低检测浓度
- 0.7 pg/mL
- 应用范围
- ELISA
- 原理
- Feline RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects Feline Rantes. Other species not determined.
- 灵敏度
- 0.7 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CCL5 ELISA Kit
- Top Product
- Discover our top product CCL5 ELISA Kit
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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鼠 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- 别名
- RANTES (CCL5 产品)
- 别名
- Rantes ELISA Kit, Scya5 ELISA Kit, D17S136E ELISA Kit, RANTES ELISA Kit, SCYA5 ELISA Kit, SIS-delta ELISA Kit, SISd ELISA Kit, TCP228 ELISA Kit, eoCP ELISA Kit, MuRantes ELISA Kit, C-C motif chemokine 5-like ELISA Kit, C-C motif chemokine ligand 5 ELISA Kit, chemokine (C-C motif) ligand 5 ELISA Kit, LOC100230935 ELISA Kit, CCL5 ELISA Kit, Ccl5 ELISA Kit
- 背景
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Gene Names: CCL5 SCYA5
Protein names: C-C motif chemokine 5 (Small-inducible cytokine A5) (T-cell-specific protein RANTES)
- 基因ID
- 493689
- UniProt
- Q8SQ40
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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