PRSS8 ELISA 试剂盒
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- 抗原 See all PRSS8 ELISA试剂盒
- PRSS8 (Protease, serine, 8 (PRSS8))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 30-8000 pg/mL
- 最低检测浓度
- 30 pg/mL
- 应用范围
- ELISA
- 原理
- Human Prostasin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4, DKK-4, DPPIV, EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR, IGFBP-5, IL-1 F5, IL-1 F6, IL-1 F7, IL-1 F8, IL-1 F9, IL-1 F10, IL-1 R5, IL-21, IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, PSMA, SIGIRR, TGF beta R3, Coagulation Factor III, TWEAK.
- 灵敏度
- 30 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling PRSS8 ELISA Kit
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- Discover our top product PRSS8 ELISA Kit
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Protease, serine, 8 (PRSS8) ELISA Kit
PRSS8 适用: 人 Colorimetric Sandwich ELISA 2.35 ng/mL - 150 ng/mL Plasma, Saliva, Serum, Tissue Homogenate, Urine
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Urine
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 人 AA 33-319 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 小鼠 AA 30-289 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 小鼠 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
Protease, serine, 8 (PRSS8) ELISA KitPRSS8 适用: 人 Colorimetric Sandwich ELISA 1560-100000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PRSS8 ELISA试剂盒
- PRSS8 (Protease, serine, 8 (PRSS8))
- 别名
- Prostasin (PRSS8 产品)
- 别名
- CAP1 ELISA Kit, PROSTASIN ELISA Kit, prostasin ELISA Kit, 2410039E18Rik ELISA Kit, AI313909 ELISA Kit, C79772 ELISA Kit, fr ELISA Kit, mCAP1 ELISA Kit, protease, serine 8 ELISA Kit, protease, serine, 8 ELISA Kit, protease, serine 8 (prostasin) ELISA Kit, PRSS8 ELISA Kit, Prss8 ELISA Kit
- 背景
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Gene Names: PRSS8
Protein names: Prostasin (EC 3.4.21.-) (Channel-activating protease 1) (CAP1) (Serine protease 8) [Cleaved into: Prostasin light chain, Prostasin heavy chain]
- 基因ID
- 5652
- UniProt
- Q16651
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