Granulin ELISA 试剂盒
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 20-15000 pg/mL
- 最低检测浓度
- 20 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse Progranulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon.
- 灵敏度
- 20 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Granulin (GRN) ELISA Kit
GRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Competition ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 AA 18-589 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Granulin (GRN) ELISA KitGRN 适用: 人 AA 18-593 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1560-100000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samples1,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
- 别名
- Progranulin (GRN 产品)
- 别名
- GRN ELISA Kit, grn ELISA Kit, wu:fb11g12 ELISA Kit, wu:fj18f05 ELISA Kit, MGC53354 ELISA Kit, DKFZp459H066 ELISA Kit, CLN11 ELISA Kit, GEP ELISA Kit, GP88 ELISA Kit, PCDGF ELISA Kit, PEPI ELISA Kit, PGRN ELISA Kit, epithelin ELISA Kit, acrogranin ELISA Kit, granulin precursor ELISA Kit, granulin a ELISA Kit, Granulins ELISA Kit, granulin L homeolog ELISA Kit, granulin ELISA Kit, GRN ELISA Kit, grna ELISA Kit, grn ELISA Kit, grn.L ELISA Kit, granulin ELISA Kit, ORF1 granulin ELISA Kit, Grn ELISA Kit
- 物质类
- Viral Protein
- 背景
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Gene Names: Grn
Protein names: Granulins (PC cell-derived growth factor) (PCDGF) (Proepithelin) (PEPI) [Cleaved into: Acrogranin (Progranulin), Granulin-1, Granulin-2, Granulin-3, Granulin-4, Granulin-5, Granulin-6, Granulin-7]
- UniProt
- P28798
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