Granulin ELISA 试剂盒
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 20-15000 pg/mL
- 最低检测浓度
- 20 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse Progranulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon.
- 灵敏度
- 20 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Granulin (GRN) ELISA Kit
GRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Competition ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 AA 18-589 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Granulin (GRN) ELISA KitGRN 适用: 人 AA 18-593 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1560-100000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samples1,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
- 别名
- Progranulin (GRN 产品)
- 物质类
- Viral Protein
- 背景
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Gene Names: Grn
Protein names: Granulins (PC cell-derived growth factor) (PCDGF) (Proepithelin) (PEPI) [Cleaved into: Acrogranin (Progranulin), Granulin-1, Granulin-2, Granulin-3, Granulin-4, Granulin-5, Granulin-6, Granulin-7]
- UniProt
- P28798
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