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GREM2 ELISA 试剂盒

GREM2 适用: 小鼠 Colorimetric Sandwich ELISA 3-800 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703418
发货至: 中国
  • 抗原 See all GREM2 ELISA试剂盒
    GREM2 (Gremlin 2 (GREM2))
    适用
    • 3
    • 2
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3-800 pg/mL
    最低检测浓度
    3 pg/mL
    应用范围
    ELISA
    原理
    Mouse PRDC ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects mouse PRDC. Other species not determined.
    灵敏度
    3 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product GREM2 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples3 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all GREM2 ELISA试剂盒
    GREM2 (Gremlin 2 (GREM2))
    别名
    PRDC (GREM2 产品)
    别名
    prdc ELISA Kit, zgc:112207 ELISA Kit, RGD1560008 ELISA Kit, Gremlin2 ELISA Kit, Prdc ELISA Kit, CKTSF1B2 ELISA Kit, DAND3 ELISA Kit, PRDC ELISA Kit, gremlin 2, DAN family BMP antagonist ELISA Kit, gremlin 2, DAN family BMP antagonist b ELISA Kit, gremlin 2, cysteine knot superfamily ELISA Kit, GREM2 ELISA Kit, grem2b ELISA Kit, grem2 ELISA Kit, Grem2 ELISA Kit
    背景
    Gene Names: Grem2 Cktsf1b2 Prdc
    Protein names: Gremlin-2 (Cysteine knot superfamily 1, BMP antagonist 2) (Protein related to DAN and cerberus) (PRDC)
    基因ID
    23893
    UniProt
    O88273
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