Periostin ELISA 试剂盒
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- 抗原 See all Periostin (POSTN) ELISA试剂盒
- Periostin (POSTN)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.05-16 ng/mL
- 最低检测浓度
- 0.05 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse Periostin ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.
- 灵敏度
- 0.05 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling POSTN ELISA Kit
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- Discover our top product POSTN ELISA Kit
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Periostin (POSTN) ELISA Kit
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Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 人 AA 22-836 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 AA 24-836 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples50 - 500 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Periostin (POSTN) ELISA试剂盒
- Periostin (POSTN)
- 别名
- Periostin (POSTN 产品)
- 别名
- fa99h07 ELISA Kit, pn ELISA Kit, postn ELISA Kit, wu:fa99h07 ELISA Kit, wu:fc70f09 ELISA Kit, zgc:153873 ELISA Kit, LOC100008945 ELISA Kit, osf-2 ELISA Kit, pdlpostn ELISA Kit, periostin ELISA Kit, OSF-2 ELISA Kit, OSF2 ELISA Kit, PDLPOSTN ELISA Kit, PN ELISA Kit, RP11-412K4.1 ELISA Kit, A630052E07Rik ELISA Kit, AI747096 ELISA Kit, Osf2 ELISA Kit, PLF ELISA Kit, peri ELISA Kit, Plf ELISA Kit, periostin ELISA Kit, periostin, osteoblast specific factor b ELISA Kit, periostin, osteoblast specific factor ELISA Kit, POSTN ELISA Kit, postnb ELISA Kit, postn ELISA Kit, Postn ELISA Kit
- 背景
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Gene Names: Postn Osf2
Protein names: Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
- 基因ID
- 50706
- UniProt
- Q62009
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