LY96 ELISA 试剂盒
LY96
适用: 人
Colorimetric
Sandwich ELISA
1.6-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all LY96 ELISA试剂盒
- LY96 (Lymphocyte Antigen 96 (LY96))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.6-400 ng/mL
- 最低检测浓度
- 1.6 ng/mL
- 应用范围
- ELISA
- 原理
- Human MD-2 (LY96) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes human MD-2. Other species not yet determined.
- 灵敏度
- 1.6 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Lymphocyte Antigen 96 (LY96) ELISA Kit
LY96 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all LY96 ELISA试剂盒
- LY96 (Lymphocyte Antigen 96 (LY96))
- 别名
- MD-2 (LY96 产品)
- 别名
- MD2 ELISA Kit, ESOP-1 ELISA Kit, MD-2 ELISA Kit, ly-96 ELISA Kit, Ly-96 ELISA Kit, Md2 ELISA Kit, lymphocyte antigen 96 ELISA Kit, LY96 ELISA Kit, Ly96 ELISA Kit
- 背景
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Gene Names: LY96 ESOP1 MD2
Protein names: Lymphocyte antigen 96 (Ly-96) (ESOP-1) (Protein MD-2)
- 基因ID
- 23643
- UniProt
- Q9Y6Y9
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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