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SCARB2 ELISA 试剂盒
SCARB2
适用: 人
Colorimetric
Sandwich ELISA
10-2500 pg/mL
Cell Culture Supernatant, Plasma, Serum
SCARB2ELISA Kit详情
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SCARB2
(Scavenger Receptor Class B, Member 2 (SCARB2))
适用
All reactivities for SCARB2 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
10-2500 pg/mL
最低检测浓度
10 pg/mL
应用范围
ELISA
原理
Human LIMPII ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
灵敏度
10 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
SCARB2目标详情
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SCARB2
(Scavenger Receptor Class B, Member 2 (SCARB2))
别名
LIMP-II (SCARB2 产品 )
别名
cd36l2 ELISA Kit, fi13c07 ELISA Kit, chunp6914 ELISA Kit, wu:fi13c07 ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, AMRF ELISA Kit, CD36L2 ELISA Kit, EPM4 ELISA Kit, HLGP85 ELISA Kit, LGP85 ELISA Kit, LIMP-2 ELISA Kit, LIMPII ELISA Kit, SR-BII ELISA Kit, 9330185J12Rik ELISA Kit, Cd36l2 ELISA Kit, MLGP85 ELISA Kit, LimpII ELISA Kit, scavenger receptor class B, member 2a ELISA Kit, scavenger receptor class B member 2 ELISA Kit, scavenger receptor class B, member 2 ELISA Kit, scarb2a ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, Scarb2 ELISA Kit
背景
Gene Names: SCARB2 CD36L2 LIMP2 LIMPII Protein names: Lysosome membrane protein 2 (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (CD36 antigen-like 2) (Lysosome membrane protein II) (LIMP II) (Scavenger receptor class B member 2) (CD antigen CD36)
基因ID
950
UniProt
Q14108
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