LAG3 ELISA 试剂盒
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- 抗原 See all LAG3 ELISA试剂盒
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 45-12000 pg/mL
- 最低检测浓度
- 45 pg/mL
- 应用范围
- ELISA
- 原理
- Human LAG-3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human LAG3. Other species not determined.
- 灵敏度
- 45 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Lymphocyte-Activation Gene 3 (LAG3) ELISA Kit
LAG3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Lymphocyte-Activation Gene 3 (LAG3) ELISA KitLAG3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphocyte-Activation Gene 3 (LAG3) ELISA KitLAG3 适用: 小鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Lymphocyte-Activation Gene 3 (LAG3) ELISA KitLAG3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples3 - 30 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all LAG3 ELISA试剂盒
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- 别名
- LAG3 (LAG3 产品)
- 别名
- CD223 ELISA Kit, LAG-3 ELISA Kit, Ly66 ELISA Kit, lymphocyte activating 3 ELISA Kit, lymphocyte-activation gene 3 ELISA Kit, LAG3 ELISA Kit, Lag3 ELISA Kit
- 背景
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Gene Names: LAG3 FDC
Protein names: Lymphocyte activation gene 3 protein (LAG-3) (Protein FDC) (CD antigen CD223)
- 基因ID
- 3902
- UniProt
- P18627
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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