Kallikrein 6 ELISA 试剂盒
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- 抗原 See all Kallikrein 6 (KLK6) ELISA试剂盒
- Kallikrein 6 (KLK6)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.6-150 ng/mL
- 最低检测浓度
- 0.6 ng/mL
- 应用范围
- ELISA
- 原理
- Human Kallikrein 6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human KLK6. Other species not determined.
- 灵敏度
- 0.6 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." in: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).
: "
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Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." in: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).
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- 抗原 See all Kallikrein 6 (KLK6) ELISA试剂盒
- Kallikrein 6 (KLK6)
- 别名
- KLK6 (KLK6 产品)
- 别名
- Bssp ELISA Kit, Klk7 ELISA Kit, PRSS18 ELISA Kit, PRSS9 ELISA Kit, SP59 ELISA Kit, hK6 ELISA Kit, Prss9 ELISA Kit, AI849898 ELISA Kit, BSP ELISA Kit, Klk29 ELISA Kit, MSP ELISA Kit, Prss18 ELISA Kit, neurosin ELISA Kit, kallikrein related peptidase 6 ELISA Kit, kallikrein related-peptidase 6 ELISA Kit, kallikrein-6 ELISA Kit, KLK6 ELISA Kit, Klk6 ELISA Kit, LOC100716801 ELISA Kit
- 背景
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Gene Names: KLK6 PRSS18 PRSS9
Protein names: Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)
- 基因ID
- 5653
- UniProt
- Q92876
- 途径
- Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling
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