Kallikrein 10 ELISA 试剂盒
-
- 抗原 See all Kallikrein 10 (KLK10) ELISA试剂盒
- Kallikrein 10 (KLK10)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-600 ng/mL
- 最低检测浓度
- 2 ng/mL
- 应用范围
- ELISA
- 原理
- Human Kallikrein 10 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human KLK10. Other species not determined.
- 灵敏度
- 2 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling KLK10 ELISA Kit
- Top Product
- Discover our top product KLK10 ELISA Kit
-
Kallikrein 10 (KLK10) ELISA Kit
KLK10 适用: 人 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kallikrein 10 (KLK10) ELISA KitKLK10 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Recommended Dilution for serum and plasma samples5 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Kallikrein 10 (KLK10) ELISA试剂盒
- Kallikrein 10 (KLK10)
- 别名
- KLK-10 (KLK10 产品)
- 别名
- KLK10 ELISA Kit, 2300002A13Rik ELISA Kit, NES1 ELISA Kit, PRSSL1 ELISA Kit, kallikrein related peptidase 10 ELISA Kit, kallikrein related-peptidase 10 ELISA Kit, kallikrein-related peptidase 10 ELISA Kit, KLK10 ELISA Kit, Klk10 ELISA Kit
- 背景
-
Gene Names: KLK10 NES1 PRSSL1
Protein names: Kallikrein-10 (EC 3.4.21.-) (Normal epithelial cell-specific 1) (Protease serine-like 1)
- 基因ID
- 5655
- UniProt
- O43240
- 途径
- Complement System
-
