IL15RA ELISA 试剂盒
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- 抗原 See all IL15RA ELISA试剂盒
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.08-20 ng/mL
- 最低检测浓度
- 0.08 ng/mL
- 应用范围
- ELISA
- 原理
- Human IL-15 R alpha ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Interleukin 15 Receptor alpha (IL15RA)
- 灵敏度
- 0.08 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 15 Receptor, alpha (IL15RA) ELISA Kit
IL15RA 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 小鼠 AA 33-205 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 小鼠 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IL15RA ELISA试剂盒
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
- 别名
- IL-15 R alpha (IL15RA 产品)
- 别名
- IL15RA ELISA Kit, CD215 ELISA Kit, AA690181 ELISA Kit, IL-15RA ELISA Kit, interleukin 15 receptor subunit alpha ELISA Kit, interleukin 15 receptor, alpha chain ELISA Kit, IL15RA ELISA Kit, Il15ra ELISA Kit
- 背景
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Gene Names: IL15RA
Protein names: Interleukin-15 receptor subunit alpha (IL-15 receptor subunit alpha) (IL-15R-alpha) (IL-15RA) (CD antigen CD215) [Cleaved into: Soluble interleukin-15 receptor subunit alpha (sIL-15 receptor subunit alpha) (sIL-15R-alpha) (sIL-15RA)]
- 基因ID
- 3601
- UniProt
- Q13261
- 途径
- JAK/STAT Signaling
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