IL-10RA ELISA 试剂盒
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- 抗原 See all IL-10RA (IL10RA) ELISA试剂盒
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2.5-500 ng/mL
- 最低检测浓度
- 2.5 ng/mL
- 应用范围
- ELISA
- 原理
- Human IL-10 R alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 2.5 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 10 Receptor, alpha (IL10RA) ELISA Kit
IL10RA 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IL-10RA (IL10RA) ELISA试剂盒
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 别名
- IL-10 R alpha (IL10RA 产品)
- 别名
- IL10R1 ELISA Kit, IL10RA ELISA Kit, CD210 ELISA Kit, CD210a ELISA Kit, CDW210A ELISA Kit, HIL-10R ELISA Kit, IL-10R1 ELISA Kit, IL10R ELISA Kit, AW553859 ELISA Kit, CDw210 ELISA Kit, CDw210a ELISA Kit, Il10r ELISA Kit, mIL-10R ELISA Kit, interleukin 10 receptor subunit alpha ELISA Kit, interleukin 10 receptor, alpha ELISA Kit, IL10RA ELISA Kit, Il10ra ELISA Kit
- 背景
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Gene Names: IL10RA IL10R
Protein names: Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
- 基因ID
- 3587
- UniProt
- Q13651
- 途径
- Growth Factor Binding
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