GREM1 ELISA 试剂盒
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- 抗原 See all GREM1 ELISA试剂盒
- GREM1 (Gremlin 1 (GREM1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.8-200 ng/mL
- 最低检测浓度
- 0.8 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse Gremlin-1 ELISA Kit for cell culture supernatants, EDTA treated plasma, and serum samples. Heparin and citrate are not recommended.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, ANG-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, IFN-gamma R1, IL-17B, IL-17B R, IL-22, MIP-1 beta, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGF beta-1, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2.
- 灵敏度
- 0.8 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Gremlin 1 (GREM1) ELISA Kit
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Gremlin 1 (GREM1) ELISA KitGREM1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Gremlin 1 (GREM1) ELISA KitGREM1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Gremlin 1 (GREM1) ELISA KitGREM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Gremlin 1 (GREM1) ELISA KitGREM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Gremlin 1 (GREM1) ELISA KitGREM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all GREM1 ELISA试剂盒
- GREM1 (Gremlin 1 (GREM1))
- 别名
- Gremlin-1 (GREM1 产品)
- 别名
- grem1 ELISA Kit, MGC136702 ELISA Kit, zgc:136702 ELISA Kit, GREM1 ELISA Kit, drm ELISA Kit, pig2 ELISA Kit, dand2 ELISA Kit, ihg-2 ELISA Kit, gremlin ELISA Kit, Cktsf1b1 ELISA Kit, Drm ELISA Kit, Grem ELISA Kit, ld ELISA Kit, gremlin-1 ELISA Kit, CKTSF1B1 ELISA Kit, DAND2 ELISA Kit, DRM ELISA Kit, GREMLIN ELISA Kit, IHG-2 ELISA Kit, cktsf1b1 ELISA Kit, gremlin 1a, DAN family BMP antagonist ELISA Kit, gremlin 1, DAN family BMP antagonist ELISA Kit, gremlin 1 ELISA Kit, gremlin 1, DAN family BMP antagonist L homeolog ELISA Kit, grem1a ELISA Kit, GREM1 ELISA Kit, LOC662541 ELISA Kit, grem1 ELISA Kit, Grem1 ELISA Kit, grem1.L ELISA Kit
- 背景
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Gene Names: Grem1 Cktsf1b1 Drm
Protein names: Gremlin-1 (Cysteine knot superfamily 1, BMP antagonist 1) (Down-regulated in Mos-transformed cells protein)
- 基因ID
- 23892
- UniProt
- O70326
- 途径
- Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location
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