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GM-CSF ELISA 试剂盒

CSF2 适用: Pig Colorimetric Sandwich ELISA 72-18.000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703063
发货至: 中国
  • 抗原 See all GM-CSF (CSF2) ELISA试剂盒
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    适用
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    Pig
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    72-18.000 pg/mL
    最低检测浓度
    72 pg/mL
    应用范围
    ELISA
    原理
    Porcine GM-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TGF beta 1, TNF alpha
    灵敏度
    72 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our best selling CSF2 ELISA Kit
    Top Product
    Discover our top product CSF2 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all GM-CSF (CSF2) ELISA试剂盒
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    别名
    GM-CSF (CSF2 产品)
    别名
    GMCSF ELISA Kit, GM-CSF ELISA Kit, CSF2 ELISA Kit, Csfgm ELISA Kit, Gm-CSf ELISA Kit, MGI-IGM ELISA Kit, Gm-csf ELISA Kit, Gmcsf ELISA Kit, CSF ELISA Kit, colony stimulating factor 2 ELISA Kit, colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit, CSF2 ELISA Kit, Csf2 ELISA Kit
    背景
    Gene Names: CSF2
    Protein names: Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF)
    基因ID
    397208
    UniProt
    Q29118
    途径
    JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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