FAS ELISA 试剂盒
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- 抗原 See all FAS ELISA试剂盒
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 40-10000 pg/mL
- 最低检测浓度
- 40 pg/mL
- 应用范围
- ELISA
- 原理
- Rat Fas (TNFRSF6/Apo-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rat FAS.
- 灵敏度
- 40 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling FAS ELISA Kit
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- Discover our top product FAS ELISA Kit
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TNF Receptor Superfamily, Member 6 (FAS) ELISA Kit
FAS 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 AA 22-169 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 人 AA 17-173 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 人 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all FAS ELISA试剂盒
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- 别名
- Fas (FAS 产品)
- 别名
- ALPS1A ELISA Kit, APO-1 ELISA Kit, APT1 ELISA Kit, CD95 ELISA Kit, FAS1 ELISA Kit, FASTM ELISA Kit, TNFRSF6 ELISA Kit, AI196731 ELISA Kit, APO1 ELISA Kit, TNFR6 ELISA Kit, Tnfrsf6 ELISA Kit, lpr ELISA Kit, Fas cell surface death receptor ELISA Kit, Fas (TNF receptor superfamily member 6) ELISA Kit, FAS ELISA Kit, Fas ELISA Kit, fas ELISA Kit
- 背景
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Gene Names: Fas Apt1 Tnfrsf6
Protein names: Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)
- 基因ID
- 246097
- UniProt
- Q63199
- 途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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