CD226 ELISA 试剂盒
CD226
适用: 人
Colorimetric
Sandwich ELISA
5-600 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- CD226
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 5-600 ng/mL
- 最低检测浓度
- 5 ng/mL
- 应用范围
- ELISA
- 原理
- Human DNAM-1 (CD226) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human DNAM1. Other species not determined.
- 灵敏度
- 5 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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CD226 (CD226) ELISA Kit
CD226 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2-20 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- CD226
- 别名
- DNAM-1 (CD226 产品)
- 别名
- CD226 ELISA Kit, cd226 ELISA Kit, zgc:158817 ELISA Kit, BC051526 ELISA Kit, DNAM-1 ELISA Kit, DNAM1 ELISA Kit, Pta1 ELISA Kit, TLiSA1 ELISA Kit, PTA1 ELISA Kit, CD226 molecule ELISA Kit, CD226 antigen ELISA Kit, CD226 ELISA Kit, cd226 ELISA Kit, Cd226 ELISA Kit
- 背景
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Gene Names: CD226 DNAM1
Protein names: CD226 antigen (DNAX accessory molecule 1) (DNAM-1) (CD antigen CD226)
- 基因ID
- 10666
- UniProt
- Q15762
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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