JAML ELISA 试剂盒
AMICA1
适用: 人
Colorimetric
Sandwich ELISA
0.41-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all JAML (AMICA1) ELISA试剂盒
- JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.41-100 ng/mL
- 最低检测浓度
- 0.41 ng/mL
- 应用范围
- ELISA
- 原理
- Human AMICA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human AMICA.
- 灵敏度
- 0.41 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all JAML (AMICA1) ELISA试剂盒
- JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))
- 别名
- AMICA (AMICA1 产品)
- 别名
- RGD1562572 ELISA Kit, AMICA ELISA Kit, CREA7-1 ELISA Kit, CREA7-4 ELISA Kit, Gm638 ELISA Kit, JAML ELISA Kit, Crea7 ELISA Kit, junction adhesion molecule like ELISA Kit, Jaml ELISA Kit, JAML ELISA Kit
- 背景
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Gene Names: AMICA1 JAML UNQ722/PRO1387
Protein names: Junctional adhesion molecule-like (Adhesion molecule interacting with CXADR antigen 1) (Dendritic cell-specific protein CREA7-1)
- 基因ID
- 120425
- UniProt
- Q86YT9
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