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人 IFN-γ ELISpot Set

ELISpot 适用: 人 Colorimetric Cell Samples Uncoated
产品编号 ABIN2690977
发货至: 中国
  • 抗原 See all Interferon gamma (IFNG) 试剂盒
    Interferon gamma (IFNG)
    适用
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    检测方法
    Colorimetric
    实验类型
    Cell ELISA
    应用范围
    ELISpot
    品牌
    BD™ ELISPOT
    样品类型
    Cell Samples
    产品特性
    The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
    组件
    10 ELISPOT plates
    Unlabeled Capture Antibody
    Biotinylated Detection Antibody
    Enzyme Conjugate
    Certificate of Analysis
    Featured
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    Discover our top product IFNG ELISA Kit
  • 应用备注
    Unique strengths of the assay include the following:
    High sensitivity
    High throughput, high content analysis
    Minimal volume of biological material required
    Applicability to frozen/thawed biological samples
    Compatibility with other assays. For example, cells analyzed by BD™ ELISPOT can be transferred for cloning, proliferation assays,flow cytometry, or other methods of analysis.
    说明

    BD™ ELISPOT Human IFN-γ ELISPOT Set

    板类型
    Uncoated
    限制
    仅限研究用
  • 储存条件
    4 °C
    储存方法
    Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.
  • Helms, Boehm, Asaad, Trezza, Lehmann, Tary-Lehmann: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).

    Power, Grand, Ismail, Peters, Yurkowski, Bretscher: "A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNgamma-producing T cells." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 99-107, (1999) (PubMed).

    Tary-Lehmann, Hricik, Justice, Potter, Heeger: "Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure." in: Transplantation, Vol. 66, Issue 2, pp. 219-24, (1998) (PubMed).

    VanCott, Staats, Pascual, Roberts, Chatfield, Yamamoto, Coste, Carter, Kiyono, McGhee: "Regulation of mucosal and systemic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1504-14, (1996) (PubMed).

    Fujihashi, McGhee, Beagley, McPherson, McPherson, Huang, Kiyono: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).

    Czerkinsky, Andersson, Ekre, Nilsson, Klareskog, Ouchterlony: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).

    Czerkinsky, Nilsson, Nygren, Ouchterlony, Tarkowski: "A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 65, Issue 1-2, pp. 109-21, (1984) (PubMed).

    Sedgwick, Holt: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).

  • 抗原
    Interferon gamma (IFNG)
    别名
    IFN-gamma (IFNG 产品)
    别名
    IFG Kit, IFI Kit, IFN-g Kit, Ifg Kit, IFNG2 Kit, IFN-gamma Kit, IFN-G Kit, IFNG Kit, IFNgamma Kit, TCRalpha Kit, INF-G Kit, ifng Kit, interferon gamma Kit, interferon, gamma 1-2 Kit, IFNG Kit, Ifng Kit, ifng1-2 Kit
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