Serotonin ELISA 试剂盒
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- 抗原 See all Serotonin (5-HT) ELISA试剂盒
- Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))
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适用
- 化学剂
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 样品类型
- Serum, Plasma, Urine, Cerebrospinal Fluid
- 灵敏度
- 5 ng/mL
- 产品特性
- Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain. Central-serotonergic neurons influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 10 μL
- 实验时间
- 2.5 h
- 板类型
- Pre-coated
- 实验流程
- The competitive Serotonin ELISA kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Serotonin (5-HT) ELISA试剂盒
- Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))
- 别名
- Serotonin (5-HT 产品)
- 物质类
- Chemical
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