IL-32 alpha ELISA 试剂盒
IL32A
适用: 人
Colorimetric
Cell Culture Cells, Plasma, Serum
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- 抗原 See all IL-32 alpha (IL32A) ELISA试剂盒
- IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))
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适用
- 人
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 32 Alpha (IL-32α) concentrations in cell culture supernates, serum, and plasma.
- 样品类型
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- 灵敏度
- 7 pg/ml
- 产品特性
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
- Top Product
- Discover our top product IL32A ELISA Kit
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Interleukin 32 alpha (IL32A) ELISA Kit
IL32A 适用: 人 Colorimetric Sandwich ELISA 15.625-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-32α ? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-32α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-32α is added to the wells and binds to the combination of capture antibody- IL-32α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-32α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-32α standard dilutions and IL-32α sample concentration determined.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IL-32 alpha (IL32A) ELISA试剂盒
- IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))
- 别名
- IL-32a (IL32A 产品)
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