IL1A ELISA 试剂盒
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma.
- 样品类型
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- 灵敏度
- 1 pg/ml
- 产品特性
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody-IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1α standard dilutions and IL-1α sample concentration determined.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL-1a (IL1A 产品)
- 别名
- IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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