Human Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit is an antibody screening test. Endothelin- receptor A has been pre-coated onto a microtiter plate. During the first incubation the anti-Endothelin receptor A-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labelled anti-human IgG4 antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-Endothelin receptor A-antibody.
应用备注
Optimal working dilution should be determined by the investigator.
样本量
100 μL
实验时间
3.5 h
实验流程
Endothelins (ET) are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. It mediates the effects through G-Protein-coupled receptors, the Endothelin receptors. There are two key receptor types, ETA and ETB. ETA receptors are found in the smooth muscle tissue of blood vessels where they increase vasoconstriction by ET-1. Human Anti Endothelin Receptor A IgG4 Antibody is designed for the determination of antibodies (IgG4) against the Endothelin receptor subtype A in serum and plasma.