This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to -sandwich- the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3-5,5- tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.