IL2RG ELISA 试剂盒
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- 抗原 See all IL2RG ELISA试剂盒
- IL2RG (Interleukin 2 Receptor, gamma (IL2RG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-10 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.054 ng/mL
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- 板类型
- Pre-coated
- 实验流程
- Interleukin 2 Receptor Gamma (IL2Rg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Gamma (IL2Rg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 2 Receptor Gamma (IL2Rg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all IL2RG ELISA试剂盒
- IL2RG (Interleukin 2 Receptor, gamma (IL2RG))
- 别名
- Interleukin 2 Receptor gamma (IL2Rg) (IL2RG 产品)
- 途径
- JAK/STAT Signaling, Growth Factor Binding
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