TIMP2 ELISA 试剂盒
(6 references)-
- 抗原 See all TIMP2 ELISA试剂盒
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 抗原表位
- AA 27-220
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat TIMP-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: C27-P220 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: C27-P220 - Featured
- Discover our best selling TIMP2 ELISA Kit
- Top Product
- Discover our top product TIMP2 ELISA Kit
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Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 马 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitSmall Sample TIMP2 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitWide Range TIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 兔 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 豚鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.
- 板类型
- Pre-coated
- 实验流程
- rat TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards(NSO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TIMP-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat TIMP-2 standard solution and each sample is measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 1.17, Standard deviation: 0.051, CV(%): 4.4
- Sample 2: n=16, Mean(ng/ml): 2.9, Standard deviation: 0.168, CV(%): 5.8
- Sample 3: n=16, Mean(ng/ml): 5.85, Standard deviation: 0.368, CV(%): 6.3,
- Sample 1: n=24, Mean(ng/ml): 1.21, Standard deviation: 0.091, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 3.55, Standard deviation: 0.256, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 6.38, Standard deviation: 0.408, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "High glucose stimulates cell proliferation and Collagen IV production in rat mesangial cells through inhibiting AMPK-KATP signaling." in: International urology and nephrology, Vol. 49, Issue 11, pp. 2079-2086, (2018) (PubMed).
: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).
: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).
: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).
: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).
: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).
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: "High glucose stimulates cell proliferation and Collagen IV production in rat mesangial cells through inhibiting AMPK-KATP signaling." in: International urology and nephrology, Vol. 49, Issue 11, pp. 2079-2086, (2018) (PubMed).
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- 抗原 See all TIMP2 ELISA试剂盒
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 别名
- TIMP2 (TIMP2 产品)
- 别名
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- 背景
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Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
Synonyms: Metalloproteinase inhibitor 2,Tissue inhibitor of metalloproteinases 2,TIMP-2,Timp2,Timp-2,
Full Gene Name: Metalloproteinase inhibitor 2
Cellular Localisation: Secreted. - 基因ID
- 29543
- UniProt
- P30121
- 途径
- cAMP Metabolic Process
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