CXCL14 ELISA 试剂盒
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- 抗原 See all CXCL14 ELISA试剂盒
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
- 抗原表位
- AA 35-111
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL14
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S35-E111 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 预测反应
- Bovine,Hamster,Horse,Monkey
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S35-E111 - Featured
- Discover our best selling CXCL14 ELISA Kit
- Top Product
- Discover our top product CXCL14 ELISA Kit
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Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA Kit
CXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 猴 AA 35-111 Colorimetric Sandwich ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Pig AA 35-111 Colorimetric Sandwich ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Hamster AA 35-111 Colorimetric Sandwich ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 马 AA 35-111 Colorimetric Sandwich ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Cow AA 35-111 Colorimetric Sandwich ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte- derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. .
- 板类型
- Pre-coated
- 实验流程
- human CXCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL14 has been precoated onto 96-well plates. Standards(E.coli, S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL14 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL14 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 987, Standard deviation: 35.5, CV(%): 3.6
- Sample 2: n=16, Mean(pg/ml): 1638, Standard deviation: 68.8, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 3285, Standard deviation: 187.2, CV(%): 5.7,
- Sample 1: n=24, Mean(pg/ml): 1387, Standard deviation: 56.87, CV(%): 4.1
- Sample 2: n=24, Mean(pg/ml): 2694, Standard deviation: 156, CV(%): 5.8
- Sample 3: n=24, Mean(pg/ml): 3525, Standard deviation: 225.6, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CXCL14 ELISA试剂盒
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
- 别名
- CXCL14 (CXCL14 产品)
- 别名
- BMAC ELISA Kit, BRAK ELISA Kit, KEC ELISA Kit, KS1 ELISA Kit, MIP-2g ELISA Kit, MIP2G ELISA Kit, NJAC ELISA Kit, SCYB14 ELISA Kit, 1110031L23Rik ELISA Kit, 1200006I23Rik ELISA Kit, AI414372 ELISA Kit, Kec ELISA Kit, MIP2gamma ELISA Kit, Scyb14 ELISA Kit, bolekine ELISA Kit, scyba ELISA Kit, fb67g04 ELISA Kit, wu:fb67g04 ELISA Kit, JSC ELISA Kit, bmac ELISA Kit, brak ELISA Kit, kec ELISA Kit, ks1 ELISA Kit, mip-2g ELISA Kit, mip2g ELISA Kit, njac ELISA Kit, scyb14 ELISA Kit, CXCL14 ELISA Kit, DKFZp469J175 ELISA Kit, C-X-C motif chemokine ligand 14 ELISA Kit, chemokine (C-X-C motif) ligand 14 ELISA Kit, chemokine (C-X-C motif) ligand 14 L homeolog ELISA Kit, CXCL14 ELISA Kit, Cxcl14 ELISA Kit, cxcl14 ELISA Kit, cxcl14.L ELISA Kit
- 背景
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Protein Function: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. .
Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.
Synonyms: C-X-C motif chemokine 14,Chemokine BRAK,MIP-2G,Small-inducible cytokine B14,CXCL14,MIP2G, NJAC, SCYB14,PSEC0212, UNQ240/PRO273,
Full Gene Name: C-X-C motif chemokine 14
Cellular Localisation: Secreted. - 基因ID
- 9547
- UniProt
- O95715
- 途径
- Autophagy
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