Corin ELISA 试剂盒
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- 抗原 See all Corin (CORIN) ELISA试剂盒
- Corin (CORIN) (Corin, Serine Peptidase (CORIN))
- 抗原表位
- AA 67-1042
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Corin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S67-N1042 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: S67-N1042 - Featured
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Corin, Serine Peptidase (CORIN) ELISA Kit
CORIN 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Corin, Serine Peptidase (CORIN) ELISA KitCORIN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Corin, Serine Peptidase (CORIN) ELISA KitCORIN 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Corin, Serine Peptidase (CORIN) ELISA KitCORIN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Corin, Serine Peptidase (CORIN) ELISA KitCORIN 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
Corin, Serine Peptidase (CORIN) ELISA KitCORIN 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S1 family.
Tissue Specificity: Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Detected in blood, in plasma as well as in serum (at protein level). .
- 板类型
- Pre-coated
- 实验流程
- human Corin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Corin has been precoated onto 96-well plates. Standards(NSO, S67-N1042) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Corin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Corin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Corin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Corin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1032, Standard deviation: 36.12, CV(%): 3.5
- Sample 2: n=16, Mean(pg/ml): 2355, Standard deviation: 89.49, CV(%): 3.8
- Sample 3: n=16, Mean(pg/ml): 3568, Standard deviation: 146, CV(%): 4.1,
- Sample 1: n=24, Mean(pg/ml): 897, Standard deviation: 42.2, CV(%): 4.7
- Sample 2: n=24, Mean(pg/ml): 2018, Standard deviation: 131.17, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 3465, Standard deviation: 249.48, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Corin (CORIN) ELISA试剂盒
- Corin (CORIN) (Corin, Serine Peptidase (CORIN))
- 别名
- CORIN (CORIN 产品)
- 别名
- CORIN ELISA Kit, CG2105 ELISA Kit, Dmel\\CG2105 ELISA Kit, SP75 ELISA Kit, LOC559109 ELISA Kit, AV273130 ELISA Kit, Lrp4 ELISA Kit, ATC2 ELISA Kit, CRN ELISA Kit, PEE5 ELISA Kit, TMPRSS10 ELISA Kit, corin, serine peptidase ELISA Kit, CG2105 gene product from transcript CG2105-RC ELISA Kit, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) ELISA Kit, corin ELISA Kit, CORIN ELISA Kit, Corin ELISA Kit, LOC559109 ELISA Kit
- 背景
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Protein Function: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.
Background: Atrial natriuretic peptide-converting enzyme, also called CRN or ATC2, is a protein that in humans is encoded by the CORIN gene. It is mapped to 4p12. This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. It has been found that Corin is the pro-ANP-converting enzyme and that Corin-mediated pro-ANP activation may play a role in regulating blood pressure. What’s more, Corin and ANP are essential for physiologic changes at the maternal-fetal interface. This protein may also function as a pro-brain-type natriuretic peptide convertase. Corin gene mutations which cause Corin to impair activity might be an underlying mechanism in hypertension.
Synonyms: Atrial natriuretic peptide-converting enzyme,3.4.21.-,Corin,Heart-specific serine proteinase ATC2,Pro-ANP-converting enzyme,Transmembrane protease serine 10,Atrial natriuretic peptide-converting enzyme, N-terminal propeptide,Atrial natriuretic peptide-converting enzyme, activated protease fragment,Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment,Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment,Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment,CORIN,CRN, TMPRSS10,
Full Gene Name: Atrial natriuretic peptide-converting enzyme
Cellular Localisation: Cell membrane, Single- pass type II membrane protein . May easily detached from the endothelial cell membrane. - 基因ID
- 10699
- UniProt
- Q9Y5Q5
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
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