IL18BP ELISA 试剂盒
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- 抗原 See all IL18BP ELISA试剂盒
- IL18BP (Interleukin 18 Binding Protein (IL18BP))
- 抗原表位
- AA 31-194
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.7-6000 pg/mL
- 最低检测浓度
- 93.7 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18BP
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: T31-G194 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: T31-G194 - Featured
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Interleukin 18 Binding Protein (IL18BP) ELISA Kit
IL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 94-6000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 20-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Tissue Specificity: Strongly expressed in heart, lung, placenta and spleen. .
- 板类型
- Pre-coated
- 实验流程
- human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1289, Standard deviation: 61.9, CV(%): 4.8
- Sample 2: n=16, Mean(pg/ml): 3561, Standard deviation: 203, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 5320, Standard deviation: 228.76, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 999, Standard deviation: 51.95, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 2895, Standard deviation: 189, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 4672, Standard deviation: 262, CV(%): 5.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all IL18BP ELISA试剂盒
- IL18BP (Interleukin 18 Binding Protein (IL18BP))
- 别名
- IL18BP (IL18BP 产品)
- 别名
- IL18BP ELISA Kit, IL-18BP ELISA Kit, Igifbp ELISA Kit, MC54L ELISA Kit, IL18BPa ELISA Kit, interleukin 18 binding protein ELISA Kit, IL18BP ELISA Kit, Il18bp ELISA Kit
- 背景
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Protein Function: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. .
Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.
Synonyms: Interleukin-18-binding protein,IL-18BP,Tadekinig-alfa,IL18BP,
Full Gene Name: Interleukin-18-binding protein
Cellular Localisation: Secreted. - 基因ID
- 10068
- UniProt
- O95998
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