Galectin 2 ELISA 试剂盒
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- 抗原 See all Galectin 2 (LGALS2) ELISA试剂盒
- Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))
- 抗原表位
- AA 1-132
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: M1-E132 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: M1-E132 - Featured
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Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA Kit
LGALS2 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA KitLGALS2 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA KitLGALS2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA KitLGALS2 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA KitLGALS2 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 2 (LGALS2) ELISA KitLGALS2 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 galectin domain.
- 板类型
- Pre-coated
- 实验流程
- human Galectin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-2 has been precoated onto 96-well plates. Standards(E.coli, M1-E132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.1, CV(%): 6.4
- Sample 2: n=16, Mean(ng/ml): 3.62, Standard deviation: 0.19, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.39, CV(%): 5.7,
- Sample 1: n=24, Mean(ng/ml): 1.48, Standard deviation: 0.13, CV(%): 8.8
- Sample 2: n=24, Mean(ng/ml): 4.12, Standard deviation: 0.3, CV(%): 7.3
- Sample 3: n=24, Mean(ng/ml): 6.54, Standard deviation: 0.49, CV(%): 7.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Galectin 2 (LGALS2) ELISA试剂盒
- Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))
- 别名
- LGALS2 (LGALS2 产品)
- 别名
- LGALS3 ELISA Kit, galectin-2 ELISA Kit, HL14 ELISA Kit, 2200008F12Rik ELISA Kit, AI324147 ELISA Kit, LGALS2 ELISA Kit, hl14 ELISA Kit, Drgal1-L3 ELISA Kit, lgals1l3 ELISA Kit, zgc:66283 ELISA Kit, Drgal1-L2 ELISA Kit, lgals1l2 ELISA Kit, zgc:86752 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose-binding, soluble 2 ELISA Kit, lectin, galactoside binding soluble 2 ELISA Kit, lectin, galactoside-binding, soluble, 2b ELISA Kit, lectin, galactoside-binding, soluble, 2a ELISA Kit, LGALS2 ELISA Kit, Lgals2 ELISA Kit, lgals2 ELISA Kit, lgals2b ELISA Kit, lgals2a ELISA Kit
- 背景
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Protein Function: This protein binds beta-galactoside. Its physiological function is not yet known.
Background: Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.
Synonyms: Galectin-2,Gal-2,Beta-galactoside-binding lectin L-14-II,HL14,Lactose-binding lectin 2,S-Lac lectin 2,LGALS2,
Full Gene Name: Galectin-2
- 基因ID
- 3957
- UniProt
- P05162
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