GAL4 ELISA 试剂盒
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- 抗原 See all GAL4 (LGALS4) ELISA试剂盒
- GAL4 (LGALS4) (Galectin 4 (LGALS4))
- 抗原表位
- AA 2-323
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-I323 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A2-I323 - Featured
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Galectin 4 (LGALS4) ELISA Kit
LGALS4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Galectin 4 (LGALS4) ELISA KitLGALS4 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Galectin 4 (LGALS4) ELISA KitLGALS4 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 4 (LGALS4) ELISA KitLGALS4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 4 (LGALS4) ELISA KitLGALS4 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Galectin 4 (LGALS4) ELISA KitLGALS4 适用: 人 Colorimetric Sandwich ELISA 0.0781-5 ng/mL Cell Culture Cells, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 2 galectin domains.
- 板类型
- Pre-coated
- 实验流程
- human Galectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been precoated onto 96-well plates. Standards(E.coli, A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-4 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.48, Standard deviation: 0.02, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 3.64, Standard deviation: 0.14, CV(%): 3.8
- Sample 3: n=16, Mean(ng/ml): 6.25, Standard deviation: 0.35, CV(%): 5.6,
- Sample 1: n=24, Mean(ng/ml): 0.51, Standard deviation: 0.03, CV(%): 5.9
- Sample 2: n=24, Mean(ng/ml): 4.02, Standard deviation: 0.19, CV(%): 4.7
- Sample 3: n=24, Mean(ng/ml): 6.93, Standard deviation: 0.43, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all GAL4 (LGALS4) ELISA试剂盒
- GAL4 (LGALS4) (Galectin 4 (LGALS4))
- 别名
- LGALS4 (LGALS4 产品)
- 别名
- LGALS4 ELISA Kit, gal4 ELISA Kit, l36lbp ELISA Kit, MGC86186 ELISA Kit, galectin-4 ELISA Kit, xgalectin-VIa ELISA Kit, gal-4 ELISA Kit, L36LBP ELISA Kit, L-36 ELISA Kit, L36LBI ELISA Kit, GAL4 ELISA Kit, galectin 4 ELISA Kit, lectin, galactoside-binding, soluble, 4 L homeolog ELISA Kit, Galectin-4 ELISA Kit, lectin, galactose binding, soluble 4 ELISA Kit, LGALS4 ELISA Kit, lgals4.L ELISA Kit, leg4 ELISA Kit, Lgals4 ELISA Kit
- 背景
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Protein Function: Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
Synonyms: Galectin-4,Gal-4,Antigen NY-CO-27,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4,LGALS4,
Full Gene Name: Galectin-4
- 基因ID
- 3960
- UniProt
- P56470
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