FCN3 ELISA 试剂盒
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
- 抗原表位
- AA 24-299
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q24-R299 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q24-R299 - Featured
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Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA Kit
FCN3 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the ficolin lectin family.
Tissue Specificity: Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells. .
- 板类型
- Pre-coated
- 实验流程
- human Ficolin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been precoated onto 96-well plates. Standards(NSO, Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-3 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 4.88, Standard deviation: 0.215, CV(%): 4.4
- Sample 2: n=16, Mean(ng/ml): 12.6, Standard deviation: 0.466, CV(%): 3.7
- Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.17, CV(%): 4.6,
- Sample 1: n=24, Mean(ng/ml): 5.14, Standard deviation: 0.35, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.02, CV(%): 7.5
- Sample 3: n=24, Mean(ng/ml): 25.7, Standard deviation: 1.98, CV(%): 7.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
- 别名
- FCN3 (FCN3 产品)
- 别名
- FCNH ELISA Kit, HAKA1 ELISA Kit, ficolin 3 ELISA Kit, ficolin-3 ELISA Kit, FCN3 ELISA Kit, Fcn3 ELISA Kit, fcn3-A ELISA Kit
- 背景
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Protein Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. .
Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.
Synonyms: Ficolin-3,Collagen/fibrinogen domain-containing lectin 3 p35,Collagen/fibrinogen domain-containing protein 3,Hakata antigen,FCN3,FCNH, HAKA1,
Full Gene Name: Ficolin-3
Cellular Localisation: Secreted. Found in blood plasma, bronchus, alveolus and bile duct. - 基因ID
- 8547
- UniProt
- O75636
- 途径
- Complement System
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