Ficolin 2 ELISA 试剂盒
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- 抗原 See all Ficolin 2 (FCN2) ELISA试剂盒
- Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))
- 抗原表位
- AA 26-313
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: L26-A313 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: L26-A313 - Featured
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Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA Kit
FCN2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA KitFCN2 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA KitFCN2 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA KitFCN2 适用: 人 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the ficolin lectin family.
Tissue Specificity: Expressed by the liver and secreted in plasma.
- 板类型
- Pre-coated
- 实验流程
- human Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO, L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 4.78, Standard deviation: 0.186, CV(%): 3.9
- Sample 2: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.648, CV(%): 5.1
- Sample 3: n=16, Mean(ng/ml): 24.1, Standard deviation: 1.11, CV(%): 4.6,
- Sample 1: n=24, Mean(ng/ml): 5.14, Standard deviation: 0.298, CV(%): 5.8
- Sample 2: n=24, Mean(ng/ml): 13.2, Standard deviation: 0.99, CV(%): 7.5
- Sample 3: n=24, Mean(ng/ml): 25.7, Standard deviation: 1.62, CV(%): 6.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Ficolin 2 (FCN2) ELISA试剂盒
- Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))
- 别名
- FCN2 (FCN2 产品)
- 别名
- EBP-37 ELISA Kit, FCNL ELISA Kit, P35 ELISA Kit, ficolin-2 ELISA Kit, Fcn2 ELISA Kit, FCN1 ELISA Kit, fcn2 ELISA Kit, fcn2.S ELISA Kit, ficolin 2 ELISA Kit, ficolin B ELISA Kit, ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ELISA Kit, ficolin 2 L homeolog ELISA Kit, FCN2 ELISA Kit, Fcnb ELISA Kit, fcn2.L ELISA Kit
- 背景
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Protein Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .
Background: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.
Synonyms: Ficolin-2,37 kDa elastin-binding protein,Collagen/fibrinogen domain-containing protein 2,EBP-37,Ficolin-B,Ficolin-beta,Hucolin,L-ficolin,Serum lectin p35,FCN2,FCNL,
Full Gene Name: Ficolin-2
Cellular Localisation: Secreted. - 基因ID
- 2220
- UniProt
- Q15485
- 途径
- Complement System
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