Renin ELISA 试剂盒
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- 抗原 See all Renin (REN) ELISA试剂盒
- Renin (REN)
- 抗原表位
- AA 67-406
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Renin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: L67-R406 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: L67-R406 - Featured
- Discover our best selling REN ELISA Kit
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Renin (REN) ELISA Kit
REN 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Renin (REN) ELISA KitREN 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Renin (REN) ELISA KitREN 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Renin (REN) ELISA KitREN 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Renin (REN) ELISA KitREN 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
Renin (REN) ELISA KitREN 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 520 Renin Assay KitFRET Fluorometric
Renin (REN) ELISA KitREN 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Renin (REN) ELISA KitREN 适用: 小鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Renin (REN) ELISA KitREN 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the peptidase A1 family.
- 板类型
- Pre-coated
- 实验流程
- human Renin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Renin has been precoated onto 96-well plates. Standards(NSO, L67-R406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Renin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Renin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Renin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 353, Standard deviation: 19.8, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 643, Standard deviation: 22.51, CV(%): 3.5
- Sample 3: n=16, Mean(pg/ml): 1204, Standard deviation: 78.26, CV(%): 6.5,
- Sample 1: n=24, Mean(pg/ml): 287, Standard deviation: 18.1, CV(%): 6.3
- Sample 2: n=24, Mean(pg/ml): 632, Standard deviation: 32.9, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 1148, Standard deviation: 81.6, CV(%): 7.1
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein." in: Hybridoma (2005), Vol. 29, Issue 6, pp. 511-6, (2010) (PubMed).
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: "Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein." in: Hybridoma (2005), Vol. 29, Issue 6, pp. 511-6, (2010) (PubMed).
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- 抗原 See all Renin (REN) ELISA试剂盒
- Renin (REN)
- 别名
- REN (REN 产品)
- 别名
- HNFJ2 ELISA Kit, RATRENAA ELISA Kit, RENAA ELISA Kit, Ren1 ELISA Kit, D19352 ELISA Kit, Ren ELISA Kit, Ren-1 ELISA Kit, Ren-A ELISA Kit, Ren1c ELISA Kit, Ren1d ELISA Kit, Rn-1 ELISA Kit, Rnr ELISA Kit, renin ELISA Kit, renin 1 structural ELISA Kit, REN ELISA Kit, Ren ELISA Kit, Ren1 ELISA Kit, ren ELISA Kit
- 背景
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Protein Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Background: Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system(RAS). This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.
Synonyms: Renin,3.4.23.15,Angiotensinogenase,REN,
Full Gene Name: Renin
Cellular Localisation: Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - 基因ID
- 5972
- UniProt
- P00797
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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